High Pure RNA Isolation Kit

RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Experiment
RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus
Product
High Pure RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
Be careful to create an RNase-free working environment
Protocol tips
Always mix the sample tube well after addition of each reagent.

Publication protocol

Total RNA was extracted from the tissues according to the manufacturer’s instructions (Spin-column, BioTeke, Beijing, China). First-strand cDNA was obtained using PrimeScript First-strand cDNA synthesis kit with Oligo dT Primer from Takara (Dalian, China). The cDNA mixture was diluted 10 times with PCR-grade water and then stored at −80 °C for qRT-PCR analysis.



Full paper   Login or join for free to view the full paper.

Reviews

High Pure RNA Isolation Kit from Roche Lifesciences has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus using High Pure RNA Isolation Kit from Roche Lifesciences.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus using High Pure RNA Isolation Kit from Roche Lifesciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms