PureLink ™ miRNA Isolation Kit

RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Experiment
RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis
Product
PureLink ™ miRNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
For retrieval of RNA from the extraction solvent following mechanical disruption, we compared simple isopropanol precipitation of RNA to solid-phase isolation of RNA using the Purelink miRNA Isolation Kit (Invitrogen, Carlsbad, CA, USA). A summary of all conditions tested and their respective nucleic acid yields before DNase I treatment as assessed by UV spectroscopy is detailed in Supplementary Table S1; the analyses were performed in three biological replicates.

Publication protocol

For retrieval of RNA from the extraction solvent following mechanical disruption, we compared simple isopropanol precipitation of RNA to solid-phase isolation of RNA using the Purelink miRNA Isolation Kit (Invitrogen, Carlsbad, CA, USA). A summary of all conditions tested and their respective nucleic acid yields before DNase I treatment as assessed by UV spectroscopy is detailed in Supplementary Table S1; the analyses were performed in three biological replicates.

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Discussion

Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis using PureLink ™ miRNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Mycobacterial RNA isolation optimized for non-coding RNA: high fidelity isolation of 5S rRNA from BCG reveals novel post-transcriptional processing and a complete spectrum of modified ribonucleosides
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink ™ miRNA Isolation Kit below.

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