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Discussion
4 years ago
Daniel McKenzie
2
Votes
2
Comments
Does anyone know how “strong” the PCR product of methylation specific PCR is? I kept my PCR products at 4C for about 3 weeks and then at room temperature for another week. Will I be able to use them for sequencing?
Experiment: PCR Methylation specific PCR
Discussion
4 years ago
R. Verma
2
Votes
2
Comments
I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?
Experiment: PCR Multiplex PCR
Discussion
4 years ago
Paul G. Macon
4
Votes
2
Comments
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Experiment: RNA isolation / purification Tissue
Discussion
4 years ago
Milena Alexeyeva
3
Votes
2
Comments
I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?
Experiment: CRISPR Human
Discussion
4 years ago
M. Daecher
2
Votes
2
Comments
Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?
Experiment: Live / Dead assay bacteria
Discussion
4 years ago
Jody Hancock
2
Votes
2
Comments
I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?
Experiment: DNA methylation profiling Gene specific profiling
Discussion
4 years ago
Aaron Stege
4
Votes
2
Comments
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Experiment: RNA isolation / purification Tissue
Discussion
4 years ago
Israel Lev
2
Votes
2
Comments
The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?
Experiment: DNA isolation / purification Bacteria
Discussion
4 years ago
Stefan Fuhrmann
1
Vote
2
Comments
I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?
Experiment: cDNA synthesis Bacteria
Discussion
4 years ago
A.C.Burton
1
Vote
1
Comment
Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA
Experiment: shRNA gene silencing Mouse