Mammalian cell culture media HCASMC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Smooth Muscle Cell Growth Medium 2

PromoCell

Upstream tips	Protocol tips	Downstream tips
	Human coronary artery smooth muscle cells were purchased from Promocell (Heidelberg, Germany) and cultured in Smooth Muscle Growth Medium Cell-2 containing 10% supplement-mix (PromoCell, Heidelberg, Germany). Cells were incubated at 37°C in a humidified atmosphere of 5% CO2. At the end of this period, the medium was removed, and cells were subjected to growth arrest in medium containing 0.1% supplement-mix before each experiment. HCASMCs between passages 3-5 were used for experiments and all explants stained positive for smooth muscle cell α-actin (Sigma, USA).

Protocol tips
Human coronary artery smooth muscle cells were purchased from Promocell (Heidelberg, Germany) and cultured in Smooth Muscle Growth Medium Cell-2 containing 10% supplement-mix (PromoCell, Heidelberg, Germany). Cells were incubated at 37°C in a humidified atmosphere of 5% CO2. At the end of this period, the medium was removed, and cells were subjected to growth arrest in medium containing 0.1% supplement-mix before each experiment. HCASMCs between passages 3-5 were used for experiments and all explants stained positive for smooth muscle cell α-actin (Sigma, USA).

Protocol tips

Human coronary artery smooth muscle cells were purchased from Promocell (Heidelberg, Germany) and cultured in Smooth Muscle Growth Medium Cell-2 containing 10% supplement-mix (PromoCell, Heidelberg, Germany). Cells were incubated at 37°C in a humidified atmosphere of 5% CO2. At the end of this period, the medium was removed, and cells were subjected to growth arrest in medium containing 0.1% supplement-mix before each experiment. HCASMCs between passages 3-5 were used for experiments and all explants stained positive for smooth muscle cell α-actin (Sigma, USA).

Manufacturer protocol Publication protocol 1 Relevant paper

SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM

Lonza

Upstream tips	Protocol tips	Downstream tips
	Human primary Coronary Artery Smooth Muscle Cells (HCASMC, Lonza #CC-2583 Lot 200,212 and Cell Applications # 350-05a Lot 1508) were cultured in Smooth Muscle Growth Medium-2 including hEGF, insulin, hFGF-B and FBS, but without antibiotics (Lonza, #CC-3182).	Cells were changed to medium with supplements at 18 h post-transfection and cultured for an additional 48 h.

Protocol tips
Human primary Coronary Artery Smooth Muscle Cells (HCASMC, Lonza #CC-2583 Lot 200,212 and Cell Applications # 350-05a Lot 1508) were cultured in Smooth Muscle Growth Medium-2 including hEGF, insulin, hFGF-B and FBS, but without antibiotics (Lonza, #CC-3182).
Downstream tips
Cells were changed to medium with supplements at 18 h post-transfection and cultured for an additional 48 h.

Manufacturer protocol Publication protocol 1 Relevant paper

Smooth Muscle Growth Supplement (SMGS)

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	HCASMC were purchased from Life technologies and cultured according to manufactures recommendation in Medium 231 supplemented with smooth muscle growth supplement (GIBCO). As per manufacturer information, cells were isolated by the enzyme digestion method.

Protocol tips
HCASMC were purchased from Life technologies and cultured according to manufactures recommendation in Medium 231 supplemented with smooth muscle growth supplement (GIBCO). As per manufacturer information, cells were isolated by the enzyme digestion method.

Manufacturer protocol Publication protocol 1 Relevant paper

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