Mammalian cell culture media RAOSMC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Minimum Essential Medium Eagle

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	A7r5 rat smooth muscle cells were obtained from the ATCC (Manassas, VA). Bovine aortic endothelial cells (BAOECs), bovine aortic smooth muscle cells (BAOSMCs), and rat aortic smooth muscle cells were obtained from Cell Applications, Inc. (San Diego, CA). Commercially available cells were grown as recommended by the supplier and exchanged into minimum Eagle's medium over time before experiments. Human brain microvascular endothelial cells were obtained from Cell Systems (Kirkland, WA) and cultured using Cell Systems complete medium before exchange into minimum Eagle's medium supplemented identically as the BAOEC culture. All vascular cells were cultured on plates or coverslips coated with 0.2% porcine gelatin (Sigma).

Protocol tips
A7r5 rat smooth muscle cells were obtained from the ATCC (Manassas, VA). Bovine aortic endothelial cells (BAOECs), bovine aortic smooth muscle cells (BAOSMCs), and rat aortic smooth muscle cells were obtained from Cell Applications, Inc. (San Diego, CA). Commercially available cells were grown as recommended by the supplier and exchanged into minimum Eagle's medium over time before experiments. Human brain microvascular endothelial cells were obtained from Cell Systems (Kirkland, WA) and cultured using Cell Systems complete medium before exchange into minimum Eagle's medium supplemented identically as the BAOEC culture. All vascular cells were cultured on plates or coverslips coated with 0.2% porcine gelatin (Sigma).

Protocol tips

A7r5 rat smooth muscle cells were obtained from the ATCC (Manassas, VA). Bovine aortic endothelial cells (BAOECs), bovine aortic smooth muscle cells (BAOSMCs), and rat aortic smooth muscle cells were obtained from Cell Applications, Inc. (San Diego, CA). Commercially available cells were grown as recommended by the supplier and exchanged into minimum Eagle's medium over time before experiments. Human brain microvascular endothelial cells were obtained from Cell Systems (Kirkland, WA) and cultured using Cell Systems complete medium before exchange into minimum Eagle's medium supplemented identically as the BAOEC culture. All vascular cells were cultured on plates or coverslips coated with 0.2% porcine gelatin (Sigma).

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