Cell line authentication

- Found 237 results

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a

RNA siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line) HSPA5 (GRP78)

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotypin

Cellular assays Cell cycle assay human AGS cell line

siRNA / miRNA gene silencing Human - Lymphoma cell line U937 MK2 (MAPK Kinase 2) Viral vectors

RNA siRNA / miRNA gene silencing Human Lymphoma cell line U937 MK2 (MAPK Kinase 2) Viral vectors

Can I use the same reagent to transfect a different protein in the same cell line?

Discussions Can I use the same reagent to transfect a different protein in the same cell line? | Labettor

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell har

Cellular assays Apoptosis assay cell type JJ012 human chondrosarcoma cell line

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell har

Cellular assays Apoptosis assay cell type L02 human hepatocyte cell line

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammali

Cellular assays Autophagy assay cell type A375 (human melanoma cell line)

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell har

Cellular assays Apoptosis assay cell type BxPC-3 human pancreatic cancer cell line

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time.

Cell culture media Mammalian cell culture media T98G

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time.

Cell culture media Mammalian cell culture media HaCaT
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